The Phosphorimager Core Facility, located in room A-022, consists of a Storm Model 860 and a Typhoon Triad. Both instruments are capable of scanning storage phosphor screens that have been exposed to radioactive samples as well as chemiluminescence and direct red or blue excited fluorescence. The Typhoon, which has greater sensitivity, can be used to scan for direct green excited fluorescence. In addition to storage screens, blots and gels can be scanned on both the Storm and Typhoon. The Typhoon is also capable of scanning a wider variety of objects, including microtiter plates, tissue sections and microarrays.
Applications include acquiring images of 1D or 2D gels, blots, tissue sections, etc. using either storage phosphor screens, chemiluminescence or specific fluorescent stains excited by red (633 nm), green (532 nm) or blue (488 or 457 nm) light. Protein or nucleic acid determination can also be done in microtiter plates when stained with relevant fluorescent dyes.
Data from any of the formats and either machine can be quantitated using available ImageQuant software.
Scheduling
Users are assigned an access code that permits entry into the facility. The machine is available at all times.
Contact
Paul Szabo, PhD
Telephone: (212) 746-8464
