Publications
Blumenthal A, Trujillo C, Ehrt S, Schnappinger D. Simultaneous analysis of multiple Mycobacterium tuberculosis knockdown mutants In Vitro and In Vivo. PLoS One 2010; 5, e15667.
Beaulieu AM, Rath P, Imhof M, Siddall ME, Roberts J, Schnappinger D, Nathan CF. Genome-wide screen for Mycobacterium tuberculosis genes that regulate host immunity. PLoS One. 2010; 5, e15120.
Kim JH, Wei JR, Wallach JB, Robbins RS, Rubin EJ, Schnappinger D. Protein inactivation in mycobacteria by controlled proteolysis and its application to deplete the beta subunit of RNA polymerase. Nucleic Acids Research 2010; Epub ahead of print.
Tan MP, Sequeira P, Lin WW, Phong WY, Cliff P, Ng SH, Lee BH, Camacho L, Schnappinger D, Ehrt S, Dick T, Pethe K, Alonso S. Nitrate respiration protects hypoxic Mycobacterium tuberculosis against acid- and reactive nitrogen species stresses. PLoS One 2010; 5 e13356.
Marrero J, Rhee KY, Schnappinger D, Pethe K, Ehrt S. Gluconeogenic carbon flow of tricarboxylic acid cycle intermediates is critical for Mycobacterium tuberculosis to establish and maintain infection. PNAS 2010; 107, 9819.
Barry CE, Boshoff H, Dartois V, Dick T, Ehrt S, Flynn E, Schnappinger D, Wilkinson RJ, Young D. The spectrum of latent tuberculosis: rethinking the biology and intervention strategies. Nature Rev Microbiol 2009; 7, 845.
Ehrt S, and Schnappinger D.& Mycobacterial survival strategies in the phagosome: defence against host stresses. Cell Microbiol 2009; 11, 1170.
Klotzsche M, Ehrt S, and Schnappinger D. Improved tetracycline repressors for gene silencing in mycobacteria. Nucleic Acid Research 2009; 37, 1778.
Vandal OH, Roberts JA, Odaira T, Schnappinger D, Nathan CF, and Ehrt S. Acid-susceptible mutants of Mycobacterium tuberculosis share hypersusceptibility to cell wall and oxidative stress and to the host environment. J Bact 2008; 191, 625.
Vandal OH, Pierini LM, Schnappinger D, Nathan CF, Ehrt S. A membrane protein preserves intrabacterial pH in intraphagosomal Mycobacterium tuberculosis. Nature Medicine 2008; 14, 849.
Boshoff HIM, Xu X, Tahlan K, Dowd CS, Pethe K, Camacho LR, Park TH, Yun CS, Schnappinger D, Ehrt S, Williams KJ, and Barry CE. Biosynthesis and recycling of nicotinamide cofactors in Mycobacterium tuberculosis: an essential role for NAD in non-replicating bacilli. J Biol Chem 2008; 28, 19329.
Gandotra S, Schnappinger D, Monteleone M, Hillen W, and Ehrt S. In vivo gene silencing identifies the Mycobacterium tuberculosis proteasome as essential for the bacteria to persist in mice. Nature Medicine 2007; 13, 1515.
Stephanoua NC, Gao F, Bongiorno P, Schnappinger D, Ehrt S, Shuman S, and Glickman MS. Mycobacterial non-homologous end joining mediates mutagenic repair of chromosomal double strand DNA breaks. J Bact 2007; 189, 5237.
Guo XV, Monteleone M, Klotzsche M, Hillen W, Braunstein M, Ehrt S, and Schnappinger D. Anhydrotetracycline-mediated silencing of the essential secA1 gene in Mycobacterium smegmatis. J Bact 2007; 189, 4614.
Morris RP, Nguyen L, Gatfield J, Visconti K, Nguyen K, Schnappinger D, Ehrt S, Liu Y, Heifets L, Pieters J, Schoolnik G, and Thompson CJ. Ancestral antibiotic resistance in Mycobacterium tuberculosis. Proc Natl Acad Sci USA 2005; 102, 12200.
Ehrt S, Guo XV, Hickey CM, Ryou M, Monteleone M, Riley LW, and Schnappinger D. Controlling gene expression in mycobacteria with anhydrotetracycline and Tet repressor. Nucleic Acids Research 2005; 33, e21.
Voskuil MI, Schnappinger D, Rutherford R, Liu Y, and Schoolnik GK. Regulation of the Mycobacterium tuberculosis PE/PPE genes. Tuberculosis 2004; 84, 256.
Voskuil MI, Schnappinger D, Visconti KC, Harrell MI, Dolganov GM, Sherman DR, and Schoolnik GK. Inhibition of respiration by nitric oxide induces a Mycobacterium tuberculosis dormancy program. J Exp Med 2003; 198, 705.
Schnappinger D, Ehrt S, Voskuil MI, Liu Y, Mangan JA, Monahan IM, Dolganov G, Efron B, Butcher PD, Nathan C, and Schoolnik GK. Transcriptional Adaptation of Mycobacterium tuberculosis within Macrophages: Insights into the Phagosomal Environment. J Exp Med 2003; 198, 693.
Shi S, Nathan C, Schnappinger D, Drenkow J, Fuortes M, Block E, Ding A, Gingeras TR, Schoolnik G, Akira S, et al. MyD88 primes macrophages for full-scale activation by interferon-gamma yet mediates few responses to Mycobacterium tuberculosis. J Exp Med 2003; 198, 987.
Krueger C, Berens C, Schmidt A, Schnappinger D, and Hillen W. Single-chain Tet transregulators. Nucleic Acids Res 2003; 31, 3050.
Ehrt S, Voskuil MI, Schoonik GK, and Schnappinger D. Genome-wide expression profiling of intracellular bacteria: The interaction of Mycobacterium tuberculosis with macrophages. Methods in Microbiology 2002; 32, 169.
Ehrt S, Schnappinger D, Bekiranov S, Drenkow J, Shi S, Gingeras TR, Gaasterland T, Schoolnik G, and Nathan C. Reprogramming of the macrophage transcriptome in response to interferon-gamma and Mycobacterium tuberculosis: signaling roles of nitric oxide synthase-2 and phagocyte oxidase. J Exp Med 2001; 194, 1123.
Sherman DR, Voskuil M, Schnappinger D, Liao R, Harrell MI, and Schoolnik GK. Regulation of the Mycobacterium tuberculosis hypoxic response gene encoding alpha-crystallin. Proc Natl Acad Sci U S A 2001; 98, 7534.
Schoolnik GK, Voskuil MI, Schnappinger D, Yildiz FH, Meibom K, Dolganov NA, Wilson MA, and Chong K H. Whole genome DNA microarray expression analysis of biofilm development by Vibrio cholerae O1 E1 Tor. Methods Enzymol & 2001; 336, 3.
Kaps I, Ehrt S, Seeber S, Schnappinger D, Martin C, Riley LW, and Niederweis M. Energy transfer between fluorescent proteins using a co-expression system in Mycobacterium smegmatis. Gene & 2001; 278, 115.
Schubert P, Schnappinger D, Pfleiderer K, and Hillen W. Identification of a stability determinant on the edge of the Tet repressor four-helix bundle dimerization motif. Biochemistry 2001; 40, 3257.
Orth P, Schnappinger D, Hillen W, Saenger W, and Hinrichs W. Structural basis of gene regulation by the tetracycline inducible Tet repressor-operator system. Nat Struct Biol 2001; 7, 215.
Schnappinger D, Schubert P, Berens C, Pfleiderer K, and Hillen W. Solvent-exposed residues in the Tet repressor (TetR) four-helix bundle contribute to subunit recognition and dimer stability. J Biol Chem & 1999; 274, 6405.
Baron U, Schnappinger D, Helbl V, Gossen M, Hillen W, and Bujard H. Generation of conditional mutants in higher eukaryotes by switching between the expression of two genes. Proc Natl Acad Sci U S A 1999; 96, 1013.
Orth P, Schnappinger D, Sum PE, Ellestad GA, Hillen W, Saenger W, and Hinrichs W. Crystal structure of the tet repressor in complex with a novel tetracycline, 9-(N,N-dimethylglycylamido)- 6-demethyl-6-deoxy-tetracycline. J Mol Biol 1999; 285, 455.
Schnappinger D, Schubert P, Pfleiderer K, and Hillen W. Determinants of protein-protein recognition by four helix bundles: changing the dimerization specificity of Tet repressor. EMBO J 1998; 17, 535.
Orth P, Alings C, Schnappinger D, Saenger W, and Hinrichs W. Crystallization and preliminary X-ray analysis of the Tet-repressor/operator complex. Acta Crystallogr D Biol Crystallogr 1998; 54, 99.
Orth P, Cordes F, Schnappinger D, Hillen W, Saenger W, and Hinrichs W. Conformational changes of the Tet repressor induced by tetracycline trapping. J Mol Biol 1998; 279, 439.
Tiebel B, Aung-Hilbrich LM, Schnappinger D, and Hillen W. Conformational changes necessary for gene regulation by Tet repressor assayed by reversible disulfide bond formation. EMBO J 1998; 17, 5112.
Berens C, Schnappinger D, and Hillen W. The role of the variable region in Tet repressor for inducibility by tetracycline. J Biol Chem 1997; 17, 6936.
Schnappinger D, and Hillen W. Tetracyclines: antibiotic action, uptake and resistance mechanisms. Arch Microbiol 1996; 165, 359.
