The NHLBI Programs of Excellence in Gene Therapy Hematopoietic Cell Processing Core (PEGT-HCPC) at the Fred Hutchinson Cancer Research Center has been established as one of six national PEGT service cores to provide large quantities of purified human or non-human primate hematopoietic stem cells to NHLBI-supported investigators (free of charge) for in vitro experimentation and/or animal model testing. This Core will obtain cells from several different hematopoietic sources. Cadaveric bone marrow will be harvested from human sources, and G-CSF mobilized peripheral blood stem cells (PBSC) will be harvested from both human and non-human primate donors. Non-human primate donors will either be baboons (Papio cynocephalus) or pig-tailed macaques (Macaca nemistrina). The Core will process those cells for CD34+ cell selection, divide the enriched cells into aliquots of 2-10 million cells, and the specimens will be cryopreserved. These purified CD34 cells will be shipped frozen to the qualified requesting investigator, free of charge. All samples will be analyzed by two or three color flow cytometry for CD34 purity and viability (purity and viability generally >98%). Non-enriched total blood components as well as the CD34-depleted flow through cells will also be cryopreserved (2-10 billion cells per bag) and can also be requested. On an individual investigator basis, additional types of cell purification (e.g. T-cell subsets, CD14+ monocytes) or provision of non-cryopreserved cells from bone marrow or PBSC can also be requested. 

For questions about custom or typical services offered by the NHLBI PEGT-HCPC, please e-mail Shelly Heimfeld, Ph.D, Fred Hutchinson Cancer Research Center, Seattle, WA at: sheimfel@fhcrc.org

To apply for services, please download the application form (requires Adobe Acrobat reader) and fax to 212-746-8824. An online form will soon be available. Applications will be reviewed on a rolling basis.