II. Endocytosis Project
The other major interest
in my lab is the study of general endocytic trafficking. Currently we are analyzing the role of
membrane cholesterol in regulating endocytosis through clathrin-coated pits and
we are studying the roles of various adaptor proteins in the regulation of
general membrane trafficking. In
these studies we use molecular cell biological methods, along with biochemical
and quantitative fluorescence microscopy.
Some recent
papers from the lab on membrane trafficking:
M Cormont, I. Metón, M.
Mari, P. Monzo, F. Keslair, C. Gaskin, T.E. McGraw and
Y. Le Marchand Brustel. 2003. D2AP / CMS regulates endosomes
morphology through its interaction with Rab4 and c-Cbl. Traffic 4:97-112.
Hu, Y., J-Z.Chuang, Kai,
Xu, T.E. McGraw, and C.H. Sung. SARA, a FYVE domain protein, affects rab5-mediated
endocytosis. 2002 J. Cell Sci. 115: 4755-4763.
Gan, Y, T.E. McGraw and E. Rodriguez-Boulan. 2002. The epithelial-specific AP1b complex mediates
post-endocytic basolateral recycling.
Nature Cell Biology, 4-605-609.
Hao,
M., S.X. Lin, O.J. Karylowski, D. Wustner, T.E. McGraw, and F.R.Maxfield. 2001. Vesicular and non-vesicular sterol transport in
living cells: the endocytic recycling compartment is a major sterol storage
organelle. Journal
Biological Chemistry, 277:609-617.
Subtil, A., I. Gaidarov,
K. Kobylarz, M.A. Lampson, J.H. Keen and T. E. McGraw. 1999.
Acute Cholesterol depletion inhibits clathrin-coated pit budding. PNAS(USA) 96: 6775-6780.